Measuring viral vector purity, stability and physical titer in minutes – with just one tool

In this webinar, Refeyn’s Laura Pala presents the KaritroMP macro mass photometer, an instrument that can quickly characterize samples of viral vectors like adenoviruses and lentiviruses. The webinar explains how macro mass photometry works and how it addresses common analytical challenges faced in viral vector production. It also shows the application of macro mass photometry to the characterization of adenovirus and lentivirus samples, and compares macro mass photometry to other commonly used techniques for viral vector characterization.

TIMESTAMPS

00:00 – Moderator introduces Laura Pala
01:33 – Laura’s talk starts
01:45 – Introduction to the KaritroMP
02:01 – Adenovirus characterization with the KaritroMP
02:11 – Analytical challenges in adenovirus production
03:42 – Why is it important to characterize adenovirus samples in process?
05:20 – How the KaritroMP helps characterize adenovirus samples
06:53 – How macro mass photometry works
09:19 – What information does the KaritroMP give you?
12:41 – The KaritroMP can assess adenovirus purity
13:40 – The KaritroMP accurately measures adenovirus empty/full ratios
16:48 – The KaritroMP can monitor adenovirus production processes
17:57 – The KaritroMP can differentiate full adenovirus from helper virus
19:40 – The KaritroMP can monitor AdV stability
21:13 – KaritroMP vs. DLS and NTA for empty/full adenovirus analysis
23:38 – Lentivirus characterization with the KaritroMP
23:46 – Analytical challenges in lentivirus production
25:04 – Why is it important to characterize lentivirus samples in process?
26:15 – How the KaritroMP helps characterize lentivirus samples
27:31 – How lentivirus samples look on the KaritroMP
29:42 – The KaritroMP can assess lentivirus purity in process
30:31 – What the KaritroMP tells us about lentivirus titer
33:17 – Consumables and workflow
36:13 – Benefits of macro mass photometry
37:21 – Conclusions

ABOUT REFEYN'S CHANNEL

Refeyn pioneers analytical instruments that put molecular mass measurement capabilities within easy reach for scientists. Refeyn instruments feature mass photometry technology, which uses light to quantify the mass of single particles in solution without labels. Find our latest video resources on YouTube, including webinars, tutorials, and much more!

ABOUT REFEYN'S CHANNEL

Refeyn pioneers analytical instruments that put molecular mass measurement capabilities within easy reach for scientists. Refeyn instruments feature mass photometry technology, which uses light to quantify the mass of single particles in solution without labels. Find our latest video resources on YouTube, including webinars, tutorials, and much more!

Check out Refeyn's channel here:
   / @refeyn  
Don’t forget to subscribe!

CHECK OUT OTHER REFEYN WEBINARS
   • Webinars  

FURTHER RESOURCES:

Application note – Quantifying adenovirus empty/full rapidly with macro mass photometry - https://info.refeyn.com/app-note-kari...

Application note – Macro mass photometry analysis of lentiviral samples correlates with titer - https://info.refeyn.com/karitromp-lvv...

Webinar – Rapid analytical approach for biologics characterization – mass photometry -    • Rapid analytical approach for biologi...  

FOLLOW REFEYN ON SOCIAL MEDIA

Twitter:   / refeynit  
LinkedIn:   / refeyn  

Hashtags
#MassPhotometry #Lentivirus #Adenovirus