Top 20 Female Spider-Man Villains

Like many of other superheroes, Peter doesn't have a very badass gallery of female villains and he's more of an ally with the one that appears most often. However, here are the most significant ones.

#20 An evil version of the spider-Woman that Dr Octopus created against Peter and the other spider-Women.
#19 Evil scientist who was originally Negative's henchwoman, has her own sidekicks named after members of the Beatles.
#18 A female Beetle and Tombstone's daughter who wants to advance her own criminal career in her father's shadow. Spider-Man villain who seems to be more of everyone else's opponent than Peter's.
#17 Rose's strong henchwoman which he used as his assassin.
#16 A dangerous seductress who uses a large snake as her weapon. The first female villain Spider-Man has faced.
#15 Ugly duck that Dr Octopus turned into a beautiful strongwoman and his sidekick and mistress.
#14 Kraven's scheming wife and mother of #7 who conjured her own husband back from the dead, eventually being killed by Kraven himself.
#13 A rapidly aging clone of Gwen that Mephisto used to try to destroy Norman and Peter.
#12 The Spider Queen, a dangerous seductress with strong telepathic abilities and sonic scream. Enemy of Spider-Man and Captain America which she transformed into the Spider King
#11 Electro's female counterpart and heir to his mantle. Member of Sinister Six/Sinister Syndicate.
#10 The fast and agile wannabe-supervillain of the social media age.
#9 Goddess of "Spider-Wasps" who hunts spider-people, practically the female equivalent of Morlun.
#8 Prison psychiatrist killed by a psychopath whose clone Norman corrupted into a female Goblin against Spider-Man.
#7 Kraven's teen daughter, female version of him, walking in her father's footsteps and continues the hunt for Spider-Man.
#6 Assassin with cybernetic enhancements who replaced Scorpion on Sinister Six and later member of Sinister Syndicate.
#5 a witch woman with voodoo magic, appeared as Kraven's lover and partner.
#4 Dangerous former drug dealer with sonic scream and flight. Appeared as Carnage's lover and partner and member of his "family".
#3 Harry's former partner which Norman turned into a female Goblin against Harry and Peter. Later she became antihero Queen Cat.
#2 Female version of Doc Ock, she replaced Otto when he was dead in the 90's. Later made a comeback, e.g. in Sinister Syndicate.

#HM1 Trapster's female version usually member of Sinister Syndicate.
#HM2 Female symbiote against whom Venom and Peter fought, mainly Venom's enemy.
#HM3 Militant feminist strongwoman originally an enemy of Peter and Tigra.
#HM4 Arcade's henchwoman which he uses to kidnap the heroes.
#HM5 Peter's part-time ally and lover, his own "Cat-Woman". basically an anti-hero who has appeared as an enemy a couple of times.

#1 the most Batman-style villain from Peter's rogues gallery and the most faced of his female enemies. Originally a rich wannabe supervillain who idolized Lewis Carroll and tends to ally with other villains against Spider-Man. Member of Sinister Syndicate and her own team The Menagerie. Later she has become a more serious villain trying to infiltrate and make a name for himself in NY crime circles.