Direct analysis of 33 amino acids in beverages by LC-MS/MS

This video presents you the direct analysis of 33 amino acids in beverages by Shimadzu LCMS8060NX with Nexera UHPLC as front end. Amino acids are building blocks of proteins and needed in many vital processes in human body. Traditional and most widely used approach for amino acid analysis is by HPLC. However, this method required derivatisation of amino acids, resulting in prolong sample preparation/analysis time. This challenge can be overcome by using LCMSMS. Direct analysis of amino acid is possible due to its high sensitivity and selectivity. In addition, UFMS technologies deliver fast scanning speed and polarity switching speed, ensuring good data quality when coupled with UHPLC. Therefore, Shimadzu Nexera UHPLC with LCMS8060NX was employed for this analysis. Tea, energy drink and red wines were subjected to amino acids analysis using this method. L-theanine was detected in tea samples. Proline was detected in chrysanthemum tea and red wines samples. Energy drink was detected with Taurine and Lysine.
#Shimadzu_LCMS
#Shimadzu_Nexera_UHPLC
#Amino_acid_analysis