Simultaneous Analysis of 10 Saccharides and Sugar Alcohols in Beverages by Nexera LC-40 and ELSD

This video presents you the simultaneous analysis and separation of 10 saccharides and sugar alcohols in beverages by Shimadzu Nexera LC-40 and ELSD. Saccharides and sugar alcohols show very narrow UV absorption wavelength range, from 190 nm to 195 nm. Therefore, a refractive index detector (RID) is commonly used for this analysis. However, gradient elution cannot be used with RID because the baseline drifting derived from the change of mobile phase composition during gradient elution is unacceptable. So, RID is not suitable for a simultaneous separation of compounds that show widely different retention behaviours due to expected long analysis time without gradient elution. Evaporative light scattering detector (ELSD) is one of universal detector that detects the scattering light from the target compounds after nebulizing and evaporating the mobile phase. ELSD provides reduced analysis time and simultaneous separation of compounds that show widely different retention due to applicability to gradient elution. The performance of Nexera LC40 and ELSD was evaluated by intra-day and inter-day repeatability tests. Good intra and inter-day reproducibility results show the analysis method and systems are very stable.

#Shimadzu
#Saccharides
#sugar
#fructose
#sucrose
#glucose
#sugar alcohols
#Analytical instrument
#Analytical Intelligence
LC40
#ELSD
#ELSD-LT III
#Advanced i-series
#food
#beverage
#column separation
#calibration curve
#Quantitative analysis
#repeatability test
#Shimadzu Asia Pacific
#HPLC
#UHPLC
#High Performance Liquid Chromatography