Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning

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Golden Gate Cloning or Golden gate assembly is a restriction enzyme based cloning that uses type IIS restriction enzymes. It is a powerful way to clone large numbers of inserts in a single reaction without the need of using multiple enzymes. This video discusses the basis of golden gate assembly or golden gate cloning, and the intuition behind using a type IIS restriction enzyme. I also discuss how you could do golden gate cloning without using Type IIS restriction enzymes. Towards the end, I discuss the syntax involved in working with golden gate assembly or cloning.

Jump to your favorite section:
00:00 Type IIS enzymes
01:16 Incompatible overhangs
01:58 How to Golden Gate
05:08 Golden Gate syntax

References/Resources:   / the_crux  

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