Protein Interactions Affects Insulin Secretion | Protocol Preview

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Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells - a 2 minute Preview of the Experimental Protocol

Charles Zhang, Arthur T. Suckow, Steven D. Chessler
University of California, San Diego, Pediatric Diabetes Research Center; Janssen Research & Development,; University of California, San Diego, Department of Medicine;

Transcellular protein interactions are important determinants of pancreatic beta-cell function. Detailed here is a method—adapted from a coculture model of synaptogenesis—for investigating how specific transmembrane proteins influence insulin secretion. Transfected HEK293 cells express proteins of interest; beta cells do not need to be transfected or otherwise directly perturbed.

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