SYBR Green qPCR Master Mix: Simplifying and Economizing Real-Time PCR | GlpBio

SYBR Green qPCR Master Mix is a pre-made mixture of reagents that is used in quantitative polymerase chain reaction (qPCR) experiments. The mix typically contains a thermostable DNA polymerase, deoxynucleoside triphosphates (dNTPs), buffer, and SYBR Green, a fluorescent dye that binds to double-stranded DNA and fluoresces when excited by a specific wavelength of light.

One of the main advantages of using SYBR Green qPCR Master Mix is that it eliminates the need for expensive, labeled probes or primers, which can be costly and time-consuming to design and synthesize. Instead, SYBR Green binds to any double-stranded DNA, making it a versatile and cost-effective option for detecting and quantifying a wide range of targets.

The SYBR Green qPCR Master Mix is added to the reaction mixture along with the template DNA and primers, and the reaction is run in a thermal cycler. As the PCR amplification proceeds, the amount of double-stranded DNA in the reaction increases, leading to an increase in the fluorescence of the SYBR Green dye. The fluorescence is measured at regular intervals during the PCR cycle, allowing for real-time quantification of the target DNA.

It's important to note that when using SYBR Green qPCR, a melting curve analysis is performed to confirm the specificity of the PCR product. This is because SYBR Green binds to any double-stranded DNA, so it is necessary to confirm that the amplified product is the specific one of interest and not a non-specific amplification product.

Overall, SYBR Green qPCR Master Mix is a convenient and cost-effective option for real-time PCR experiments, as it eliminates the need for labeled probes or primers, and allows for easy quantification of a wide range of targets.

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