How to do a hematology stain (Wright-Giemsa stain) Laboratory Tutorial Procedure

Wright-Giemsa Stains, also known as Romanowsky Stains, are stains used in hematology and cytological studies to differentiate cells in microscopic examinations of peripheral blood and bone marrow specimens.

These stains can also be applied to detect the presence of parasites in the blood when rapid results are needed, such as for the detection of the Plasmodium parasite that causes malaria.

There are a variety of Romanowsky stain types that apply the same principle. They include:
1. Giemsa stain
2. Wright and Wright-Giemsa stain
3. May-Grunwald stain
4. Leishman stain

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00:00 Intro
00:09 History of Wright-Giemsa stains
02:49 Preparing specimen smears
03:12 Basic blood slide preparation
04:48 Prepare reagents for the Wright-Giemsa stain
06:18 Performing the Wright-Giemsa stain
06:40 Step 1 Fix slide with methanol
07:06 Step 2 Place slide in Wright-Giemsa Stain
07:29 Step 3 Remove and drain slide
07:39 Step 4 Place slide in buffer
07:57 Step 5 Remove and drain slide again
08:09 Step 6 Place slide in rinse solution
08:34 Step 7 Dry slide
08:44 Step 8 View slide under microscope
09:04 Table guide
09:20 Ordering info
09:46 Hardy Diagnostics Close

Filmed and Edited by Brian Tom