How to design antisense oligonucleotides for high on-target potency and minimal off-target effect

Описание к видео How to design antisense oligonucleotides for high on-target potency and minimal off-target effect

Antisense oligonucleotides (ASOs) are synthetic nucleotides that are designed to modulate gene expression. Learn more at: https://idtb.io/brongh

There are different types of ASOs with different mechanisms of action. RNase H1 gapmer ASOs are chemically modified and designed to cleave their target RNAs. Steric blocking ASOs, are often designed to block the splicing machinery to alter splicing outcomes, or to block the ribosome to inhibit translation.

By watching this video, you’ll pick up insightful tips on gapmer ASOs, which use the endogenous RNase H1 enzyme to degrade a target RNA.

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