How to optimize CRISPR genome editing in primary T cells

Описание к видео How to optimize CRISPR genome editing in primary T cells

Genome editing in primary T lymphocytes has unique challenges that make generating correct clones a significant bottleneck in the progression of research. This video presents optimized methods for CRISPR editing in primary T cells including optimized cell culture and delivery parameters for high-efficiency editing. Additionally, we discuss methods for improving knock-in rates with both short and long inserts through optimized design of HDR donors and the use of HDR enhancing reagents. Learn more at https://idtb.io/r2880a

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