Elucidate Bacterial Colonization Factors In Burkholderia gladioli Symbiont l Protocol Preview

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Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles - a 2 minute Preview of the Experimental Protocol

Ramya Ganesan, Martin Kaltenpoth, Laura V. Flórez
Johannes Gutenberg University, Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution; Max Planck Institute for Chemical Ecology, Department of Insect Symbiosis; University of Copenhagen, Department of Plant and Environmental Sciences, Section for Organismal Biology;

This is an adapted method for identifying candidate insect colonization factors in a Burkholderia beneficial symbiont. The beetle host is infected with a random mutant library generated via transposon mutagenesis, and library complexity after colonization is compared to a control grown in vitro.

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