TOPO Cloning - TOPO-Blunt, TOPO-TA, TOPO-directional

References/Resources:   / the_crux  

All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)  

TOPO Cloning (Topoisomerase-based cloning) is a commonly used method to clone PCR amplicons. TOPO Cloning bypasses the need of restriction enzymes and ligases. In this video, we discuss the three main types of TOPO-cloning systems - TOPO Blunt, TOPO TA, and TOPO directional. TOPO Cloning is best understood through the mechanism of topoisomerase action, so the video also familiarizes you with the biochemical reaction in this cloning procedure.

Jump to your favorite section:
00:00 Mechanism
04:50 TOPO Blunt
08:28 TOPO TA
10:53 TOPO Directional
13:44 TOPO Expression Vector

References/Resources:   / the_crux  

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