Golden Gate Assembly Domestication Tutorial

Описание к видео Golden Gate Assembly Domestication Tutorial

This video explains how to perform Golden Gate Domestication, or the removal of Type IIS cut sites naturally occurring in vector or insert sequences.

0:00 - Overview of Golden Gate Assembly
0:38 – Avoiding Internal Type IIS Restriction Sites
1:02 - What is Golden Gate Domestication?
1:11 – 3 ways to manage Golden Gate Domestication
1:19 - Method 1: Fragment and Vector Selection
1:30 - Method 2: Site-Directed Mutagenesis
1:50 - Method 3: Silent Mutation

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