cDNA Synthesis Protocol by Reverse Transcription

Описание к видео cDNA Synthesis Protocol by Reverse Transcription

Hey Friends,
cDNA (complementary DNA) is synthesized from an RNA template. This is done by Reverse Transcription. In this example, the oligo d(T)-primers are used to prime the cDNA synthesis. An alternative would be to use random hexamers as primers. cDNA is synthesized before qPCR to check gene expression levels.

qPCR:
   • qPCR (real-time PCR) protocol explained  

References:
Recommended: https://www.nature.com/articles/nmeth...

More:
https://geneticeducation.co.in/revers...
https://www.ncbi.nlm.nih.gov/pmc/arti...

Cheers
Henrik

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