DNA Isolation from plant tissue

DNA: To isolate DNA from plant tissue:
• Kiwi plant is peeled and then chopped (knife with cuts away from the body) to breakdown cell walls (disrupt cell structures). Safety goggles and lab coat worn for protection.
• The chopped kiwi is added to a solution containing salt (clumps the DNA) and washing up liquid (breaks down cell membrane). This solution is then added to a water bath of 60°C (Denatures enzymes that would breakdown DNA) for 15 mins only (so DNA itself doesn’t denature).
• The solution is removed from the water bath and placed in an ice bath for 5 mins (to ensure the DNA itself doesn’t break down).
• The solution is then blended for 3 secs (to ensure cell contents are broken down but the DNA itself doesn’t breakdown) this solution is then filtered to remove the cell debris. Protease enzyme is then added to the filtered solution to break down proteins associated with DNA (DNA is packaged with protein).
• Some of this solution is added to a test tube and ice-cold ethanol is trickled down the side (to form a layer on top).
• The DNA precipitates out of solution as it is insoluble in ice-cold ethanol.
• The DNA is then removed using a glass rod (looks like white threads).