Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer

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This video discusses the plant cloning vectors focusing on Agrobacterium Tumefaciens based vectors and its dependency on the natural infection mechanism. This involves understanding Ti plasmids, disarmed Ti vectors, cointegrate vectors, and binary vectors. The process of cloning in plants uses conjugative transfer of T DNA through conjugation between agrobacterium and plant cells.

Jump to your favorite section:
00:00 Crown Gall Disease
00:47 Ti plasmid structure
02:24 T DNA transfer
03:37 Disarmed Ti vectors
05:32 Cointegrate vectors
07:51 Triparental mating
11:10 Binary vectors


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