RNA to cDNA Conversion + Amplification

Once the viral genetic material purification is completed by the extraction system, samples can be immediately transferred into a Green Button Go Pre-PCR Workcell where the RNA of the SARS-CoV-2 virus is annealed and then converted to a more stable cDNA form. Through partnership with SPT Labtech, the cDNA material is partitioned, and the necessary reagents dispensed (master mixes for each directional strand template) to accomplish this conversion using the Mosquito liquid handler and Dragonfly liquid dispenser. Once in cDNA form, the sample material can then be amplified by thermal cyclers.

Green Button Go Scheduler orchestrates the compression of sample input plates and sample tracking while automating the workflow and handling reagent stability restrictions. Samples are processed in the most efficient timeframe while preserving the consistency of each process, thereby ensuring the highest robustness and integrity.

Learn more about how Biosero’s solutions have enabled genome surveillance at scale for the COVID-19 pandemic: https://www.biosero.com/application/g...

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Biosero, Inc. develops automation solutions to help laboratories make better decisions in less time, using more data. Our integration services match laboratory automation to your science, overseeing implementation from start to finish. Coupled with Biosero Green Button Go® software, we create a cohesive technology ecosystem that accelerates operations and increases productivity. On its own, Green Button Go software is an end-to-end laboratory management solution that integrates with business process automation to keep workflows and operations moving in life science, biotechnology, pharmaceutical, and diagnostic research.
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