Demonstration of Fraction Collection in Preparative HPLC Analysis

Welcome to our comprehensive guide on Preparative HPLC and the Shimadzu fraction collector. Throughout this video, we'll explore basic concept of preparative chromatography, covering topics ranging from fundamental principles to practical operation using LabSolutions software. Typically comprising a pump, injector (manual or auto injector), column, detector, and a fraction collector, preparative HPLC serves a distinct purpose compared to its conventional counterpart. Unlike normal HPLC, preparative HPLC specializes in isolating and purifying target compounds from mixed samples, such as synthesized drugs or natural product extracts, for subsequent evaluation and analysis.
In this tutorial, we'll show the process of HPLC method development and purification techniques using LabSolutions. You'll learn how to set-up HPLC method for accurately separating and detecting compounds like caffeine and methyl paraben in a standard mixture. Additionally, we'll demonstrate how to set up a purification method using LabSolutions alongside a Preparative HPLC system equipped with a fraction collector FRC-10A. Finally, we'll guide you through processing and reporting the purification results using LabSolutions' intuitive report templates. Join us as we uncover the secrets of HPLC method development and purification with LabSolutions, empowering you to optimize your chromatography workflow with precision and efficiency.
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