To investigate the effect of denaturation on enzyme activity:

To investigate the effect of denaturation on enzyme activity:
The enzyme used in the three experiments focused on the rate of activity is Catalase which is obtained from celery, its substrate is Hydrogen Peroxide its product is oxygen (seen as foam in the graduated cylinder as the oxygen is trapped by the washing up liquid) and water.
All three experiments are set up using the same volume of catalase solution (obtained by blending some celery), pH buffer and a few drops of washing up liquid in a graduated cylinder. The same volume of hydrogen peroxide is then added to the graduated cylinder and the rate of activity is measured by tracking the volume of foam produced per min after adding the hydrogen peroxide
• Two samples of catalase solution are taken, one is left as normal (control) the other is boiled for 5 mins.
• The temperature (use a water bath) and pH (using a pH buffer) are kept the same for both solutions.
Result: The enzyme that was boiled had no activity (denatured i.e. no foam) the other sample worked to it’s optimum (large volume of foam produced).